Characterization and profiling of the microRNA in small extracellular vesicles isolated from goat milk samples collected during the first week postpartum

Colostrum contains nutrients, immunoglobulins, and various bioactive compounds such as microRNA (miRNA). Less is known about the temporal changes in miRNA profiles ruminant milk samples during first week postpartum. In this study, we characterized compared of small extracellular vesicles (sEV) isolated from colostrum (CM, collected immediately after parturition, n = 8) transition (TM, 7 d postpartum, eight 1-yr-old Guanzhong dairy goats with a yield approximately 500 kg/year. A total 192 unique sEV-associated (transcripts per million >1 at least 4 either CM or TM) were identified all samples. There 29 uniquely TM while no was The abundance top 10 accounted for 82.4% ± 4.0% (± SD) abundance, let-7 families (e.g., let-7a/b/c-5p) being predominant predicted to target 1,008 genes that may regulate pathways focal adhesion, TGF-β signaling, axon guidance. expression patterns EV similar between 2 sample groups, although let-7c-5p miR-30a-3p higher, whereas let-7i-5p miR-103–3p lower than TM. conclusion, core miRNAome play an important role cell proliferation, bone homeostasis, neuronal network formation newborn goat kids. lack differential be due relatively short sampling interval which diet composition, intake health status ewes, environment stable.